By Y. Kitagawa, T. Matsuda, S. Iijima

Animal mobilephone expertise is a growing to be self-discipline of mobile biology which goals not just to appreciate constructions, capabilities and behaviors of differentiated animal cells but in addition to envision their skills for use for commercial and scientific reasons. The aim of animal mobilephone expertise contains accomplishments of clonal enlargement of differentiated cells with necessary skill, optimization in their tradition stipulations, modulation in their skill for creation of medically and pharmaceutically very important proteins, and the appliance of animal cells to gene remedy and synthetic organs. This quantity provides the readers a whole evaluation of the current cutting-edge in Japan. The lawsuits might be worthwhile for mobilephone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines relating to animal mobilephone tradition, operating both in educational environments or in industries of biotechnology and pharmacy.

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W. CHO Department of Biology, Yonsei University 134 Shinchon-Dong, Seodaemun-Ku, Seoul 120-749, Korea 1. Polysialic Acid-containing Glycoproteins in the Jelly Coat of Sea Urchin Eggs and the Sea Urchin Egg Receptor for Sperm A gelatinous jelly coat sea urchin eggs consists of a mixture of Sia-rich glycoproteins and fucose-rich glycoproteins [1]. Chemical and physical studies recently revealed the presence of a novel polysialic acid (polySia) structure in the sea urchin glycoproteins isolated from Hemicentrotous pulcherrimus, designated polySia-gp(H) and Stronylocentrotus purpuratus designated polySia-gp(S) [2].

Expression Cloning of Two Types of cDNAs We constructed an expression cloning system using both an Epstein-Barr virus-based cloning vector pAMo and Burkitt lymphoma cell line Namalwa KJM-1 as a host. To isolate glycosyltransferases involved in the biosynthesis of the determinants, we developed an expression cloning system using lectin-resistance selection. Ricinus communis agglutinin is a cytotoxic lectin specific to galactose residues, especially structure, which is a backbone of the structure.

1991) MHCrestricted recognition of autologous melanoma by tumor-specific cytotoxic T cells evidence for restriction by a dominant HLA-A allele. J. Immunol. 146, 1692-1699. , and Gazdar, A. , USA. A. (1994) Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection. Proc. Natl. Acad. Sci. USA. 91, 6458-6462. , and Ohno, T. (1995) Induction of human autologous cytotoxic T lymphocytes on formalin-fixed and paraffin-embedded tumour sections.

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