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Since most organic oxidations are only two-electron processes, higher sensitivity is typically found for nitro compounds. Several LCEC based determination of nitro compounds have been reported 232- 239) 26 Electrochemical Techniques in Bioanalysis Table 4. Applications of LCEC to drug analysis Class Compound Structure Ref. 267) 268) 269) 270) 271) 272) Heterocycles: Several heterocycles of biomedical interest are reducible. A m o n g these the K vitamins 240,241) and the pterins 241) have been determined by LCEC.

Procedures using both preformed films and in situdeposited films have been used extensively. 0 V vs SCE from an acidic Hg 2 ÷ solution. The electrode is then removed from the plating solution and used for sample analysis. In situ-deposited films are formed simultaneously with deposition of sample metals by adding a small amount of Hg 2 ÷ to all standard and sample solutions. The mercury film is removed after each analysis either by wiping it off or by anodically stripping it to soluble Hg 2+, in the absence of anions which may cause precipitation.

And J. S. ) Marcel Dekker: NewYork, 1981) 39 Craig E. Lunte and William R. Heineman the electrode to give a satisfactory stripping signal in much shorter times. Since the deposition is not exhaustive, it is important to deposit the same fraction of analyte for each stripping voltammogram in a series of standards and samples. The parameters of electrode surface area, deposition time, and stirring must be carfully duplicated for all standards and samples. Deposition times vary from 60 sec to 30 rain, depending on analyte concentration, type of electrode, and stripping technique.

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