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6) Optionally stain the grids with uranyl acetate and/or lead citrate. Results can be improved by treating sections collected in plastic rings. The sections are then deposited onto a grid at the very end of the treatment. In all cases copper grids have to be avoided (risk of oxidation during the incubation with the gold complex creating metallic deposits). C. Roland and B. Vian Gold particle Enzyme Substrate (a) Tissue —v section , Gold particle Gold particle Protein with specific affinity for the lectin Lectin M M M y Sugar residue M Tissue section (b) (c) Fig.
Trials Prior to Ultrastructural labelling The development of affinity methods presents good opportunities for the combination of structural (LM) and ultrastructural (EM) observations. At the LM level this labelling has several advantages such as: first, the confirmation of the fidelity of localization by the survey of whole tissues at low magnification and, second, the ability 1. General Preparation and Staining of Thin Sections 27 to utilize the same metallic markers on both LM and EM sections.
15. Illustration following a PATAg test. Cotyledon of tamarind seed. The polysaccharides react strongly: starch in the plastids (p) and the cell walls (w). The latter are specially thick and massive, being hypertrophied by storage of xyloglucan; n, nucleus ( x 4000). 1. General Preparation and Staining of Thin Sections a >^y~< / oo 41 *-r-0 -0 J 1* 4 , I ; I oo\ooj oo I oo n 1 oo I oo I oo Fig. 16. Contrasting response of polysaccharides to the same cytochemical PATAg test, (a) 1-4 linkage, numerous vic-glycols available and possibility of strong reaction, (b) 1-3 linkage, no vic-glycol available except the terminal, and thus almost no possibility of periodic oxidation and no visible reaction.
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