By John R. Masters (Editor), Bernhard O. Palsson (Editor), James A. Thomson (Editor)

If you want to develop or signify embryonic stem cells or convince them to distinguish right into a specific mobilephone sort, then this publication comprises info that's very important on your good fortune. the purpose is to supply transparent easy directions and protocols for becoming, retaining and characterizing embryonic stem cells and information of a few of the tools used to make stem cells differentiate into particular telephone forms. The contents should be of curiosity to stem telephone biologists, tissue engineers and scientists wishing to exploit embryonic stem cells for healing reasons. every one bankruptcy has been written and edited by means of the world over revered scientists operating on the leading edge of technological advancements in human embryonic stem cells.

Show description

Read or Download Embryonic Stem Cells (Human Cell Culture) PDF

Best microbiology books

Guidelines for Drinking-Water Qualtiy Addendum: Microbiological Agents in Drinking-Water

This Addendum to the second one variation of the tips for drinking-water caliber comprises reports of a few pathogens that happen commonly in water - Aeromonas and Legionella spp. , Vibrio cholerae, enteric hepatitis viruses, and protozoan parasites (Cryptosporidium, Giardia, and Cyclospora spp. ).

Prescott's Principles of Microbiology

Prescott’s rules of Microbiology maintains within the culture of the industry major Prescott, Harley, and Klein’s Microbiology. In utilizing the seventh version of PHK’s Microbiology because the origin for the improvement of ideas, the authors have offered a streamlined, briefer dialogue of the extensive self-discipline of microbiology and feature desirous about clarity and the mixing of numerous key subject matters with an emphasis on evolution, ecology and variety in the course of the textual content.

Microarray detection and characterization of bacterial foodborne pathogens

It is a assessment of contemporary advances at the use of DNA microarray for diagnosing foodborne pathogens. quick detection and characterization of foodborne pathogens is necessary for foodstuff protection. Many appropriate applied sciences were intensively built up to now. DNA microarray expertise bargains a brand new approach to nutrients defense related to pathogen detection and characterization.

Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 2

This publication evaluation sequence provides present tendencies in glossy biotechnology. the purpose is to hide all elements of this interdisciplinary know-how the place wisdom, equipment and services are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computing device technology. Volumes are prepared topically and supply a finished dialogue of advancements within the respective box over the last 3-5 years.

Extra resources for Embryonic Stem Cells (Human Cell Culture)

Sample text

Many of the antibodies used in the characterization of hESC lines are directed either against cell surface epitopes expressed at discrete stages of mouse embryonic development or against epitopes displayed on human embryonal carcinoma cells. The stage-specific embryonic antigens 1, 3, and 4 are globoseries glycolipids recognized by monoclonal antibodies originally raised by immunization of mice with embryos. While mouse ES (mES) cells are characterized by expression of SSEA-1, hESC express SSEA-3 and SSEA-4, and express SSEA-1 only upon differentiation.

2003). In principle, a wide range of non-human oocytes should support successful SCNT of human cells, including rabbit, sheep, pig, and cow oocytes, which have already been shown to support intra-species SCNT. The latter three species are particularly useful as there are large numbers of ovaries available from these species from abattoirs that could be used for human SCNT. The enucleation process appears to be crucial for propagation of the embryo following nuclear transfer. , Simerly et al. 2003).

TARGETED GENETIC MODIFICATION VIA HOMOLOGOUS RECOMBINATION Historically, researchers have either analyzed naturally occurring mutants, or applied random mutagenesis strategies and studied the resulting phenotypes. However, although these experiments have yielded many interesting insights over the years, their outcomes vary widely and generally defy prediction. In the modern laboratory setting, it is more desirable to create specific and controlled genetic modifications. In recent years, homologous recombination has been used to alter genomes in living cells (Smithies et al.

Download PDF sample

Rated 4.19 of 5 – based on 32 votes