By Apurba Sankar Sastry and Sandhya Bhat

The subsequent new release approach-one weapon, ambitions: a unmarried booklet that meets a number of requisites- MBBS second yr examination and PG front tests • extra content material, much less pages-handy glance, saves a student’s time

• Concise, bulleted layout and to-the-point text-easy to learn in the course of exam

• uncomplicated and lucid language makes the certainty effortless

• Separate packing containers for precis of laboratory prognosis and treatment-for fast overview

• Schematic diagrams are drawn in a effortless demeanour

• extra genuine images-keeping in view the image-based MCQs in PG entrances

• adequate tables and flowcharts-for higher realizing

• Immunology and virology sections-totally a brand new glance given, absolutely up to date and in keeping with the present desire

• contemporary up to date options in epidemiology, laboratory prognosis and remedy incorporated

• complicated and more recent subject matters lined, equivalent to H1N1, Ebola, Polio eradication, more recent bacterial drug resistance mechanisms (ESBL, VRSA, VRE, XDR-TB) automations and molecular equipment in microbiology, and so forth.

• scientific case-based essay questions and MCQs integrated on the finish of the chapter-to make stronger the scholars to organize for destiny tests.

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E. g. Campyloliacter and Helicoliacter). g. C/0Slridit1m). stolyticttm). g. carbon) for its survival and reproduction. Bacterial metabolism can be based on diree principles: 1. How the bacteria obtain carbon for synthesizing cell Organisms 1ha1 require higheramoun1s of carbon dioxide (510%) for growdi are called capnophllic bacteria. Examples include Brttcella abortus, Streptococct1s pneumonia/\ etc. e. human body temperature). However, die optimal temperature range varies widi differem bacterial species.

Cell wall of gram-negative bacteria, which gels disrupted easily by die action of acetone or alcohol; thus allowing die primary stain to come ' out of the cytoplasm. 0 0 After mordanting with Gram's iodine, bigger dyeC:::::::::, Step-1: Primary stain iodine complexes are formed in the cytoplasm. Crystal violet for 1 minute Following decolorizalion, as more lipid content ~ Water rinse in gram-negative bacterial cell wall ge1s dissolved , . xes escape. _ Step-2: Mordant Gram's iodine for 1 minute to less lipid in gram-positive bacterial cell wall smaller pores are formed which do nm allow 1he / 0 0 Water rinse dye- iodine com pl exes 10 escape .

But can revert back to the original morphology once penicillin is removed. • Protoplasts: They are gram--positive bacteria whose ce ll wall isentirely removed • Spheroplasts: They are derived from gram--negatiYe bacteria whose cell wall ispartially removed. • Stable L f orms: L forms that are unable to revert to the original bacteria are called stable L forms . • Mycop/a smod o not have a true cell wall; the peptidoglycan layer is rep laced by sterol. • It is postulated that Mycop/osmo may represent stable L forms of a yet to be identified parent bacteria.

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