By P. Michael Conn (Eds.)
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This Addendum to the second one version of the ideas for drinking-water caliber comprises experiences of a few pathogens that take place greatly in water - Aeromonas and Legionella spp. , Vibrio cholerae, enteric hepatitis viruses, and protozoan parasites (Cryptosporidium, Giardia, and Cyclospora spp. ).
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6 1 . T . Frielle, K. W . Daniel, M . G. C a r o n , and R. J. Lefkowitz, Proc. Natl. Acad. Sci. A. 85, 9494 (1988). 62. A . K a m b , L . E . I v e r s o n , and M . A . T a n o u y e , Cell 50, 405 (1987). 63. D . M . P a p a z i a n , T . L . S c h w a r z , B . L . T e m p e l , Y . N . J a n , and L . Y. J a n , Science 237, 749 (1987).  Xenopus OOCYTE EXPRESSION 29 64. B . L . T e m p e l , D . M . P a p a z i a n , T. L . S c h w a r z , Y. N . J a n , and L . Y. J a n , Science 237, 770 (1987).
H e r t z b e r g and R. G. ), " G a p J u n c t i o n s . " L i s s , N e w Y o r k , 1988. 57. T. T a k a h a s h i , M . K u n o , M . Mishina, and S. N u m a , J. Physiol. (Paris) 80, 229 (1985). 58. R. J. L e o n a r d , C. G. L a b a r c a , P . C h a r n e t , N . D a v i d s o n , and H . A. L e s t e r , Science 242, 1578 (1988). 59. K . I m o t o , C. B u s c h , B . S a k m a n n , M . Mishina, T. K o n n o , J. N a k a i , H . Bujo, Y. M o r i , K . F u k u d a , a n d S. N u m a , Nature (London) 335, 645 (1988).
In the case of the recording pipette, a low resistance minimizes the noise introduced into the clamp. For the current electrode, a low resistance is needed so that large currents may be injected into the oocyte. Currents of tens of microamperes are sometimes required to charge rapidly the membrane capacitance when stepping the membrane potential to activate voltage-gated channels, and similarly large ligand-gated currents may be induced by potent m R N A preparations. In practice, the tip size of the pipettes is a matter of compromise; the electrical properties of the pipettes improve with increasing size, but more damage is done to the oocyte on impalement.
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