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All rights of reproduction in any form reserved. ISBN 0-12-406080-3 24 H U M A N LYMPHOKINES these experiments. Preparation of Lymphotoxin: Lymphotoxin was prepared by 2 different techniques as described below: (a) White blood cells were obtained from the interferon laboratory of Wadley Institutes of Molecular Medicine after the cells had been in tissue culture for 24 hours in the presence of Sendai virus and the supernate containing interferon had been harvested (12). The remaining cells were then washed in MEM Hank's medium.
JONES: We haven't looked at either of those. It certainly does not occur in the absence of plasminogen, so it is plasminogen dependent. AGGARWAL: Would you like to speculate the importance of this plasminogen activator which is induced by interferon? " AGGARWAL: What is the physiological evidence, if any? JONES: I think this has already been mentioned. It may have a role in hemostasis, although I doubt at the concentrations we are talking about, if it really has any role at all. What the role is in terms of host defense, I don't know.
In spite of the enormous popularity of this assay system, results could only be obtained by taking precautions. In figures 1 and 2 the influence of pH-value and osmotic pressure is demonstrated. e. pH 7,2 - 7,6 and around 0,15 M NaCl is a good locomotion in controls observed. % inhibition 6 7 8 9 10 PH FIGURE 1. Influence of different pH-value s on the randan migration of the target-cells % inhibition 500 osmotic pressure 1 000 1 500 ( mosmol ) FIGURE 2. Influence of osmotic pressure on the random migration of the target cells I.
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