By S. H. Gillespie (Auth.)
This full-colour, hugely illustrated textual content relates laboratory bench way to medical perform. It covers the proper specimens to ship to the laboratories, how those will be processed, which pathogens should be remoted and alternative ways during which they could be pointed out
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Additional info for Medical Microbiology Illustrated
A phospholipase is elaborated which may assist in breaking down host membranes and Listeria along with other Gram-positive organisms possesses a cholesterol binding ('thiol activated') toxin listerio-lysin, analogous to streptolysin-O and pneumolysin. Clinical importance Listeriosis may give rise to a number of different clinical syndromes. These include acute meningoencephalitis, especially in immunocompromised and elderly patients. Neonatal infection takes two forms: the early-onset disease Listeria are readily isolated from clinical specimens, such as blood, cerebrospinal fluid (CSF), amniotic fluid, genital tract secretions, or biopsies.
A strip of filter paper soaked in antiserum is placed into the medium as it solidifies. Test and control strains are streaked at right angles and after growth toxin diffuses from the streaks to form a precipitin line with the antitoxin diffusing from the strip. A line of identity with the positive control confirms the diagnosis of toxigenic C. diphtheriae Precipitation will take place between antitoxin diffused from the strip and toxin produced by the organism (Fig. 6). 35 identification of Enterobacteriaceae, may be employed with the addition of one or two drops of fetal calf or horse serum.
Group I consisted of organisms which were pigmented when exposed to light, the photochromogens. Group II were organisms which were pigmented when incubated in the dark, the scotochromogens. Organisms which were not capable of pigment production were placed in Group III (non-chromogens) and the rapid growers were in Group IV. This classification has little clinical relevance and pigmentation can be variable in some organisms, notably M. avium-intracellulare and M. szulgai. 1). This also provides a guide to laboratory examination and clinical advice.
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