By Guillermo López-Campos, Joaquín V. Martínez-Suárez, Mónica Aguado-Urda, Victoria López-Alonso
This is a overview of modern advances at the use of DNA microarray for diagnosing foodborne pathogens. swift detection and characterization of foodborne pathogens is important for nutrition protection. Many suitable applied sciences were intensively constructed so far. DNA microarray know-how bargains a brand new method to nutrients defense regarding pathogen detection and characterization. DNA microarray can be utilized for detection and characterization of pathogens via studying hybridization styles among trap probes and nucleic acids remoted from foodstuff samples or micro organism. It permits extra quick, exact, and competitively priced detection of pathogens in comparison with conventional techniques of cultivation or immuno-assays. the applying of DNA microarrays to various foodborne micro organism, comparable to Campylobacter, Salmonella, Listeria monocytogenes, or Shiga toxin generating Escherichia coli, will enhance their speedy identity and characterization in their genetic features (e.g., antimicrobial resistance, virulence). As bacterial foodborne ailments are posing extra severe threats to public healthcare, improvement of speedy and actual equipment for pathogen detection and characterization is necessary to their right regulate on the earliest time.
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This can be a assessment of modern advances at the use of DNA microarray for diagnosing foodborne pathogens. swift detection and characterization of foodborne pathogens is necessary for nutrients security. Many appropriate applied sciences were intensively built so far. DNA microarray know-how bargains a brand new option to foodstuff safeguard concerning pathogen detection and characterization.
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Extra info for Microarray detection and characterization of bacterial foodborne pathogens
D’Haese E, Nelis HJ (2002) Rapid detection of single cell bacteria as a novel approach in food microbiology. J AOAC Int 85:979–983. Favrin SJ, Jassim SA, Griffiths MW (2003) Application of a novel immunomagnetic separationbacteriophage assay for the detection of Salmonella Enteritidis and Escherichia coli O157: H7 in food. Int J Food Microbiol 85:63–71. Fedio WM, Jinneman KC, Yoshitomi KJ, Zapata R, Wendakoon CN, Browning P, Weagant SD (2011) Detection of E. coli O157:H7 in raw ground beef by Pathatrix™ immunomagneticseparation, real-time PCR and cultural methods.
Multiplexing is accomplished by using different microsphere sets based on color (Miller and Tang 2009). 2 DNA Microarray Principles and Technologies 39 The current generation of commercially available suspension bead arrays (LabMap System, Luminex) offers a 100-element array that uses a flow cytometer for analysis of the microspheres in suspension. The flow cytometer provides a method to simultaneously detect DNA-binding events on each microsphere. Two spectrally distinct fluorophores are incorporated on polystyrene microspheres (FlowMetrix Beads).
A custom Affymetrix microarray can be quite expensive, and the inflexibility of its custom mask makes the use of an Affymetrix-synthesized array impractical for some applications. In contrast, the NimbleGen and Agilent platforms are easily customized with a unique oligonucleotide sequence content. In addition, a webbased tool provided by Agilent called eArray allows users to design custom microarrays with no minimum manufacturing batch size requirements, making Agilent microarrays a primary choice for homebrew and pilot applications.
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