By A C Thaysen And L D Galloway

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This is usually reached after 96 hours' incubation at the optimum temperature. At this stage the fungus covers the culture medium with a densely matted layer of mycelial growth containing numerous sporangia. The accumulation of enzyme in the medium is now particularly rapid, and the spent culture medium, with its salts and decomposition products, is therefore replaced by distilled water, into which the enzyme continues to diffuse. An aqueous solution of the enzyme is thus obtained and can be concentrated by evaporation in vacuo.

Soc. Chem. , vol. 46, p. 279 T, 1927. 14. W. Nageli, Die StdrJeekorner, Zurich, 1868. 15. A. Meyer, Untersuchungen -uber die St&rkekbrner, Jena, 1895. 16. Z. Gatin-Gruzewska, Comytes rend. soc. , vol. 64, p. , vol. 146, p. 640,1908. 17. C. , vol. 168, p. 1353,1914; vol. 159, p. 630,1914. 18. H. Pringaheim and K. Wolfsohn, Benchte, vol. 67, p. 887,1024. 19. M. Sameo, EoUoiddiern. Betiiefie, vol. 4, p. 23,1914. 20. E. , vol. 144, p. 146,1907-8; Intern. Cong. Appl. Chem. London, vol. 7, VI. B. Fermentation, p.

Vol. 144, p. 146,1907-8; Intern. Cong. Appl. Chem. London, vol. 7, VI. B. Fermentation, p. 129,1909. {M. Sameo and F. v. Hoefift, EoUoidchem. Beihefte, vol. 4, 132,1912; vol. 6, p. 141, 1913; vol. 6, p. 201, 1014. M. Sameo and H. Haerdtl, Kottoidchem. Beihefte, vol. 12, p. 281,1920. M. Sameo and A. Meyer, gottoidchem. Beihefte, vol. 16, p. 89,1922. M. Samec, Kottoidchem. , voL 47, p. 81,1929. 22. D. H. F. Clayaon and S. B. Scnryver, Biochem. , vol. 17, p. 403,1923. 23. S. B. Sohryver and E. M.

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