By W. SIMPSON

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The answers are 102-a, 103-c, 104-b, 105-e, 106-d. ) Hepatitis A virus (HAV) possesses a single-stranded linear RNA genome while hepatitis B virus (HBV) contains a doublestranded DNA genome. Detection of anti-HAV IgM in a single serum specimen obtained in the acute or convalescent stage is the quickest and most reliable method to diagnose hepatitis A infection. This antibody is usually present at onset of symptoms and may persist 3 to 6 months. Demonstration of hepatitis B surface antigen (HBsAg) in serum is the most common method of diagnosing HBV infection.

Koplik’s spots, pale, bluish-white spots in red areolas, can frequently be observed on the mucous membranes of the mouth and are pathognomonic for measles. 30. The answer is e. ) The definitive diagnosis of rabies in humans is based on the finding of Negri bodies, which are cytoplasmic inclusions in the nerve cells of the spinal cord and brain, especially in the hippocampus. Negri bodies are eosinophilic and generally spherical in shape; several may appear in a given cell. Negri bodies, although pathognomonic for rabies, are not found in all cases of the disease.

Louis encephalitis, yellow fever, and dengue are caused by flaviviruses. Western equine encephalitis is caused by an alphavirus. Laboratory diagnosis is usually made by demonstration of a fourfold rise in specific antibody titer in paired sera. 37. The answer is a. ) Ribavirin is effective to varying degrees against several RNA- and DNA-containing viruses in vitro. qxd 40 11/14/01 4:59 PM Page 40 Microbiology tions in infants. Intravenous administration has proved effective in treating Lassa fever.

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