By Alain Filloux, Juan-Luis Ramos
In Pseudomonas aeruginosa, professional researchers within the box aspect the various equipment that are now customary to review this attention-grabbing microorganism. Chapters comprise microbiological the right way to high-throughput molecular suggestions which have been constructed during the last decade. Written within the hugely profitable Methods in Molecular Biology sequence structure, chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, comfortably reproducible laboratory protocols and key pointers on troubleshooting and keeping off recognized pitfalls.
Authoritative and useful, Pseudomonas aeruginosa aids within the carrying on with examine of recent and leading edge findings.
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Extra info for Pseudomonas Methods and Protocols
7. Count the plaques formed in the drop of the dilution with 3–30 bacteriophage plaques. 8. Calculate the titre of the bacteriophage of triplicate preparations using the Eq. 1. 6 Concentration and Purification of Bacteriophage Lysates This protocol was adapted from Sambrook and Russell ; however, it includes some minor modifications and does not include an ultracentrifugation step. 1. To a 250 mL bottle, add 100 mL of bacteriophage lysate, and DNase I and RNase A (1 μg/mL each). 2. Incubate for 30 min at room temperature.
5 ml Eppendorf tube containing lysozyme buffer. Incubate 2 h at 37 °C. Discard the lysozyme buffer. Add 1 ml ES + proteinase K and continue with step 10. 6. If agarose blocks do not harden in the mold, the agarose concentration is too low.
1007/978-1-4939-0473-0_4, © Springer Science+Business Media New York 2014 23 24 Joana Azeredo et al. extracellular polymeric substances (EPS) depolymerase within the bacteriophage genome. Bacteriophages specific for Pseudomonas were first described in mid-twentieth century [3, 4] and, to date, are known for only little over a dozen of the more than 230 described Pseudomonas species . , application on patients with chronic otitis and venous leg ulcers) [14, 15], among others. Due to its pathogenicity, bacteriophages targeting P.
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